![]() ![]() Chemicals and other consumables are warranted through their expiration date, or for 1 year from shipment if no expiration date is indicated.Repairs have different warranty periods.Instrument warranties do not include consumable parts, such as lamps, lasers, and platinum wire. Warranty periods are listed on quotations for specific products however, instruments generally receive 1 year of warranty coverage from the date of shipment or installation.Should a Bio-Rad branded product fail to meet specifications during its warranty period, it shall be repaired or replaced at Bio-Rad's discretion. View our full Standard Terms and Conditions of Sale at For more information, contact your local Bio-Rad Customer Care office at Return PolicyĪll Bio-Rad products are guaranteed to meet the specifications listed in our catalog. Delivery terms (Incoterms 2010) shall be set forth in any Quotation. Unless otherwise agreed, Buyer shall clear any imported Goods at the point of import and pay all relevant duties. Each installment shall be invoiced and considered a separate sale. Goods may be shipped, depending on lead time and availability, in installments. Prepare 0.Current Listings 111 LabX Member Since 28th August, 2014 (9 years ago) Seller Location Shipping PolicyĪll Goods shall be suitably packed in Bio-Rad's standard shipment packaging, marked, and shipped in accordance with Bio-Rad’s applicable specifications (or if no specifications are provided, in accordance with reasonable commercial practices) using a carrier of Bio-Rad’s choice. Mix the Clarity Western ECL Substrate Kit components in a 1:1 ratio. It is important to use an ECL substrate that has good sensitivity and long signal duration, such as the Clarity Western ECL Substrate. After the final wash step, keep the blot in TBST while preparing for blot detectionĪll PrecisionAb Antibodies were validated using enhanced chemiluminescent (ECL) detection. Rinse the blot with 15 ml TBST at RT for 5 min. Incubate the blot in the secondary antibody and blocking buffer solution at RT for 1 hr with gentle agitation Please refer to the antibody product page for details on the exact secondary antibody used during the validation process. Repeat for a total of five washesĭilute the appropriate secondary antibody in 10 ml blocking buffer according to the following table: Incubate the blot in the primary antibody and blocking buffer solution at 4☌ overnight with gentle agitation Please see the validation protocol (bulletin 6603) for more details.ĭilute the primary antibody 1:1,000 in 10 ml blocking buffer If using BSA, you may notice some nonspecific bands due to its low stringency. We recommend using casein or nonfat dried milk for blocking. When using casein, do not block for longer than 30 min to prevent reduction in signal specificity. Load the control cell lysate adjacent to your samples and the molecular weight (MW) marker (see diagram).ĭuring the validation process, we blocked for 30 min at room temperature (RT) in blocking buffer + 0.1% Tween 20. If using BME, add 180 μl H20, 200 μl 2x Laemmli Sample Buffer, and 20 μl BME If using DTT, add 190 μl H20, 200 μl 2x Laemmli Sample Buffer, and 10 μl 2 M DTT Reconstitute 400 µg lysate in one of the following ways, depending on the reducing reagent used: Secondary antibodies (see antibody datasheet) Trans-Blot Turbo Mini PVDF Transfer Pack.Transfer membranes, reagents, and equipment 1x Tris/glycine/SDS (TGS running buffer). ![]() Precision Plus Protein All Blue Standards Value Pack.Mini-PROTEAN Tetra Cell for Mini Precast Gels. ![]()
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